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MuViScopy: This new microscope set up for 3D live imaging is now published in Development

labex deep
02/21/2022
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MuViScopy: a new microscope setup enabling full 3D live imaging at cellular.
This was a great collaboration between the Curie imaging facility PICT-IBiSA@BDD and the teams of Yohanns Bellaïche (Institut Curie, UMR3215/U934) and Philippe Girard (Institut Jacques Monod).


Temporal projection fly
Color coded temporal projection of a timelapse movie of a developing fly during metamorphosis, acquired from three views (dorsal, lateral and ventral) using MuViScopy (Multi-View confocal microScopy).

MuViScopy: a new microscope setup for live imaging, enabling full  multi-organ and whole organism 3D live imaging at cellular level without need for embedding.

This si the result of a great collaboration between the Curie imaging facility PICT-IBiSA@BDD and the teams of Yohanns Bellaïche (Institut Curie, UMR3215/U934) and Philippe Girard (Institut Jacques Monod).

The article in now published in the journal Development. Congratulations to all authors!

 

ABSTRACT

Understanding how development is coordinated in multiple tissues and gives rise to fully functional organs or whole organisms necessitates microscopy tools. Over the last decade numerous advances have been made in live-imaging, enabling high resolution imaging of whole organisms at cellular resolution. Yet, these advances mainly rely on mounting the specimen in agarose or aqueous solutions, precluding imaging of organisms whose oxygen uptake depends on ventilation. Here, we implemented a multi-view multi-scale microscopy strategy based on confocal spinning disk microscopy, called Multi-View confocal microScopy (MuViScopy). MuViScopy enables live-imaging of multiple organs with cellular resolution using sample rotation and confocal imaging without the need of sample embedding. We illustrate the capacity of MuViScopy by live-imaging Drosophila melanogaster pupal development throughout metamorphosis, highlighting how internal organs are formed and multiple organ development is coordinated. We foresee that MuViScopy will open the path to better understand developmental processes at the whole organism scale in living systems that require gas exchange by ventilation.