Actualité - Events

Naama Barkai, Weizmann Institute of Science, member of the DEEP SAB

Seminar LabEx DEEP - Nuclear Dynamic Series

Tuesday, January 19th 2021 1:00 pm

Naama Barkai, Weizmann Institute of Science; LabEx DEEP Scientific Advisory Board (SAB) Member

«Strategies of nucleosome exchange»
Naama Barkai


Seminar LabEx DEEP - Nuclear Dynamic Series

Tuesday, January 19th 2021
1:00 pm

Centre de recherche - Paris Webinar

Naama Barkai, Weizmann Institute of Science
LabEx DEEP Scientific Advisory Board (SAB) Member

«Strategies of nucleosome exchange»

Histone exchange shapes the epigenetic landscape and regulates DNA-related processes.  In the exchange processes, histones present at particular DNA locations (and carry position-dependent marks) are replaced by histones from the free pool. Currently, studies of histone exchange are limited by time delays and temporal perturbations inherent to pulse-chase histone labeling. To overcome these limitations, I will describe an “exchange timer” –an endogenous marker that is modified at each genomic locus according to the local histone exchange rate. Using this sensor, we followed the exchange of core histones in budding yeast during steady growth and upon stress exposure. Based on  our results, I will discuss the possibility that nucleosome subcomplexes are exchanged independently. I will further describing data pointing at distinct exchange strategies, which depend on either active transcription, or the potential for transcriptional changes.


Selected recent publications:

Independent evolution of transcript abundance and gene regulatory dynamics. Genome Res. 2020 Jul;30(7):1000-1011. doi: 10.1101/gr.261537.120. Epub 2020 Jul 22. DOI: 10.1101/gr.261537.120

Intrinsically Disordered Regions Direct Transcription Factor In Vivo Binding Specificity.Mol Cell. 2020 Aug 6;79(3):459-471.e4. doi: 10.1016/j.molcel.2020.05.032. Epub 2020 Jun 16. DOI: 10.1016/j.molcel.2020.05.032

Transcription Factor Binding to Replicated DNA. Cell Rep. 2020 Mar 24;30(12):3989-3995.e4. doi: 10.1016/j.celrep.2020.02.114. DOI: 10.1016/j.celrep.2020.02.114

A repressor-decay timer for robust temporal patterning in embryonic Drosophila neuroblast lineages. Elife. 2018 Dec 10;7:e38631. doi: 10.7554/eLife.38631. DOI: 10.7554/eLife.38631

Epigenetic Control of Expression Homeostasis during Replication Is Stabilized by the Replication Checkpoint. Mol Cell. 2018 Jun 21;70(6):1121-1133.e9. doi: 10.1016/j.molcel.2018.05.015. Epub 2018 Jun 14. DOI: 10.1016/j.molcel.2018.05.015


Invited by:

Geneviève Almouzni, Institut Curie

Pierre Leopold, Institut Curie



Participer à la réunion Zoom

Meeting ID: 964 9375 8652

Passcode: 546648